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#Macsquant facs flowjo update#

Fully utilize fluorescent proteins and reporter applications that require the Violet, Blue or Yellow laser such as mCherry, GFP and CFP.

It is a perfect match for labs utilizing fluorescent protein reporters or for labs that want to simply use PE and FITC conjugates.įinding the right fit for fluorescent proteins with the yellow laser On top of the performance, automation, compactness and convenience associated with the MACSQuant ® Family of flow cytometers, the MACSQuant VYB comes with a uniquely configured optical bench, featuring violet, yellow, and blue lasers. All in all my impression from using it the last few years is that it is a great machine for all our routine 3-4 colour work. But I guess this is why this machine is essentially 1/4 the price?! One nice feature is that it gives you a cell concentration as well because it takes a certain volume for each sample rather than continuously aspirate until it gets the number of stated events. Do note that the resolution of populations is not as good as a high end BD machine. You do need to do Miltenyi Transform to see the cells but otherwise that's the only tweak required.

#Macsquant facs flowjo update#

They do come and update the software now and again but this also means you lose your data so you need to back this up.Īnalysis: we do all this in FlowJo. Only once did we have serious blockage and at one stage one of the valves started to leak. Service: we have had no serious issues with the machine. The FACS also has a mix option as well so there is no problem with the cells settling in the bottom of the well either. Note - if you do not have the optional refilling station the running buffer will run out about 3/4 of the way through so you need to be there to replace it. It can also do high throughput and we routinely run 96 well plates. We also do four colours very simply when we have mCherry which is detected in the Y2 channel. Miltenyi has a great selection of antibodies as well. We have the three laser version and there is no real need for compensation if you use the vioBlue, FITC (GFP) and then the Y4 (PE/APC-Vio770 conjugated antibodies) channels. Our use is mainly characterization of hematopoietic cells and the occassional adherent cell line. It goes from 9am to 4pm non-stop most days. " For the MACsQuant or the Vyb as we call it, is a solid work horse of the lab.

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